NEXome Core Panel

Catalog Number:

#1001852（16 rxn）
#1001851（96 rxn）

On the basis of the latest version of Refseq (109, 2021) and the CCDS database, NEXome Core Panel has carefully selected some regions that are worthy of attention outside the database. At the same time, based on product continuity considerations, some egions deleted in the latest version of the database have been retained. The NEXome Core Panel contains approximately 400,000 single-stranded DNA probes by independently synthesized and independently inspected for quality, targeting a 34.7Mb genomic region (19,613 genes). NEXome Core Panel, as a core all-exome Panel which can be combined with different spike-in panels to meet different application requirements.

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Product details
Kit Content
FAQ
Ordering Information
Resources

Production and Quality Control

Independently Synthesized and Independently Inspected for Quality

Fig 1. Mass spectrometry results of a single probe from NEXome Core Panel. The theoretical molecular weight was 37542.9 Da, and the mass spectrometry results showed that the measured molecular weight was 37538.6 Da.

Table 1. Examples for analysis detection of each probe.

Performance

Capture Performance

Fig 2. Capture performance of the NEXome Core Panel in different gDNA libraries
A. Mappability and On-target rate, B. Target covered,C. Coverage uniformity and consistency, D. The unbiased of GC coverage;300 ng gDNA NadPrep^® DNA libraries were prepared using the NadPrep DNA Universal Library Preparation Kit Series, NEXome Core Panel (6 plex) were respectively used to complete hybridization capture. The BWA was used for alignment of raw reads to the reference genome (hg38). Sequencing platform: Illumina Novaseq 6000，PE150；DNBSEQ-T7，PE150.
Note: Male (Promega-Male, G1471); Female (Promega-Female, G1521)

Variant Analysis

Fig 3. Correlation between expected allele frequency and observed allele frequency.

DNA libraries were prepared using NadPrep DNA Universal Library Preparation Kit Series and NEXome Core Panel were respectively used to complete hybridization capture. VarDict was used for variant calling. Sequencing platform: Illumina Novaseq 6000，PE150；DNBSEQ-T7，PE150.

Note: Sample type: PancancerLight 800 gDNA Reference Standard with 100 ng (Genewell, GW-OGTM800).

Product Advantage

Fig 4. Performance comparison of different whole exome panel for on-target rate and target covered of gDNA libraries.。

A. On-target；B. Target covered；C. Depth of coverage;The BWA was used for alignment of raw reads to the reference genome (hg38). Sequencing platform: Illumina Novaseq 6000，PE150

Fig 5.The achievable coverage depth of target area for different whole exome panel under the same amount of sequencing data.、

Fig 6. Coverage of NEXome Core Panel

Catalog#	Color Of Tube Cap	Item	Volume	Package/Storage
1001852		NEXome Core Panel, 16 rxn	70 μL	–20℃
1001851		NEXome Core Panel, 96 rxn	415 μL	–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.