NadPrep Multigene Methyl Control

Catalog Number:

#1005541

NadPrep Multigene Methyl Control series are region-specific controls designed specifically for multigene methylation detection. All the Controls comprise 7 tumor-associated methylation targets with established research and clinical application backgrounds. The product includes negative, low-, and high-methylation levels, establishing a gradient-based, gene-specific methylation control system. The materials are derived from human cell lines and feature an authentic and stable genomic background, with well-characterized methylation levels, and excellent batch-to-batch consistency. They can closely mimic methylation patterns in clinical specimens, providing reliable and stable support for quality assessment and performance veriﬁcation of diﬀerent methylation detection systems.

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Gene List

Target Gene	Covered Region (hg19)	Associated Cancer	Selection Basis
RPRM	chr2:154335130-154335200	Gastric cancer	NMPA-approved
RNF180	chr5:63461576-63461701	Gastric cancer	NMPA-approved
SDC2	chr8:97506002-97506575	Colorectal cancer, gastric cancer	NMPA-approved
BCAT1	chr12:25101871-25102163	Colorectal cancer	NMPA-approved
NDRG4	chr16:58497348-58497506	Colorectal cancer	FDA- and NMPA-approved
SEPTIN9	chr17:75368886-75369820	Colorectal cancer/gastric cancer, esophageal cancer	FDA- and NMPA-approved
PAX1	chr20:21686414-21686513	Cervical cancer	NMPA-approved

Feature

● Selected Targets · Clinical Relevance

Selected from clinically relevant targets covered by FDA/NMPA-approved methylation detection products, covering 7 representative methylation targets associated with colorectal, gastric, cervical, and esophageal cancers (247 CpG loci). Supporting QC for multigene and multicancer methylation detection.

● Gradient Design · Comprehensive QC

Includes negative, low-, and high-methylation levels to establish a gradient-based QC system. Multiple validations enable comprehensive QC across different methylation levels and support multidimensional evaluation of detection performance, including accuracy and reproducibility.

● Human Genomic Background · Realistic Simulation

Uses carefully selected human cell line-derived materials with stable target gene expression. The target regions exhibit authentic and stable methylation patterns, better simulating methylation patterns in clinical specimens and offering representative QC materials.

● Batch-to-batch Stability · Reliable Results

Offers excellent batch-to-batch consistency and reliable quality for stable long-term supply. Suitable for quality monitoring across different experimental batches and long-term testing process, helping ensure consistent and reliable detection results.

● Broad Compatibility · Flexible Applications

Compatible with multiple methylation detection methods, including bisulfite conversion, methylation-sensitive restriction enzyme (MSRE)-based methods, methylation-specific quantitative PCR (MethyLight), methylation-specific PCR (MSP), and pyrosequencing for method verification and performance evaluation.

Performance

Comprehensive Evaluation Across Methylation Levels

Figure 1. Quantitative accuracy assessment of all CpG loci within the target regions of NadPrep Multigene Methyl Control series.

Note：20 ng of fragmented NadPrep Multigene Methyl Control series were used for pre-library preparation with NadPrep Methyl Library Preparation Module v2 and NadPrep Methyl Stubby Adapter (UDI) Module v2 (for Illumina®), followed by bisulﬁte conversion using NadPrep DNA Methyl Bisulﬁte Conversion Module. A total of 500 ng of each pre-library was used for hybrid capture with μCaler Hybrid Capture Reagents v2 and μCaler EMS Panel v1.0.

Broad Coverage of Cancer-Associated Targets

Figure 2. Quantitative detection results of methylation targets associated with multicancer.

For research use only. Not for use in diagnostic procedures.

Sub-catalog#	Color of Tube Cap	Item	Scale 1005541(1 μg/tube，3 tubes/box)
1005541-1		NadPrep Multigene Methyl Negative Control	1 μg
1005541-2		NadPrep Multigene Low-Methyl Positive Control	1 μg
1005541-3		NadPrep Multigene High-Methyl Positive Control	1 μg

For research use only. Not for use in diagnostic procedures.

Type	Product	Catalog#	Scale	Item	Sub-catalog#	Mass
QC	NadPrep Multigene Methyl Control	1005541	1 μg/tube， 3 tubes/box	NadPrep Multigene Methyl Negative Control	1005541-1	1 μg
				NadPrep Multigene Low-Methyl Positive Control	1005541-2	1 μg
				NadPrep Multigene High-Methyl Positive Control	1005541-3	1 μg