NadPure Blood Genomic DNA Kit

Catalog Number:

#1010101
#1010102

NadPure Blood Genomic DNA Kit utilizes high-binding superparamagnetic beads and a specially optimized buﬀer system to rapidly isolate and purify high-quality genomic DNA from 50-1,000 μL of fresh or frozen blood samples containing various anticoagulants.
The kit provides a safe and convenient extraction workﬂow without the use of toxic reagents such as phenol or chloroform. It eﬀectively removes proteins, inorganic salts, and other contaminants while maintaining high nucleic acid recovery. The puriﬁed nucleic acids can be directly used for a variety of downstream applications, including PCR, qPCR, RT-PCR, Southern blotting, genotyping, and the library preparation of next-generation sequencing.

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Kit Content
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Ordering Information
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Featuer

Magnetic Separation, Reduced Equipment Dependence: Magnetic handling eliminates the need for a large centrifuge, reducing equipment dependence and simplifying the process.

Simple and Fast, Improved Efficiency: Optimized extraction workflow delivers ultra-pure gDNA in under 55 min, enhancing sample processing efficiency.

Selective Binding, Efficient Purification: Selective gDNA binding enables efficient removal of contaminants such as hemoglobin, minimizing downstream inhibition.

Gentle Extraction, Preserved Integrity: Gentle extraction and elution conditions minimize DNA damage and fully protect the integrity of gDNA.

Consistent Performance, Reliable Batch-to-Batch Quality: Stable extraction performance across batches, with a CV well below 15%, helps ensure consistent nucleic acid quality.

Robust Compatibility, Ultra-High Throughput: Compatible with challenging blood samples, including hemolyzed, lipemic, and anticoagulated samples, and supports ultra-high-throughput automation.

Workflow

Performance

Flexible Compatibility Across Sample Input Volumes

Figure 1. Performance of the NadPure Blood Genomic DNA Kit for gDNA extraction form the whole blood at different input volumes. A. DNA Yield; B. DNA Purity; C. Agarose gel electrophoresis of extracted gDNA (100 ng loaded per lane; the same loading amount applies to subsequent figures unless otherwise specified).

Broad Compatibility with Samples of Varying Quality

Figure 2. Comparison of the extraction performance among different magnetic bead-based blood gDNA extraction kits using various types of blood samples. A. DNA yield; B. DNA purity; C. Agarose gel electrophoresis of extracted gDNA. gDNA was extracted from fresh and hemolyzed whole blood samples using the NadPure Blood Genomic DNA Kit and comparable kits from Vendor A & B, respectively.
Note: The initial sample input volume was 200 μL for all samples. FWB: Fresh whole blood; HWB: Hemolyzed whole blood.

Reliable Capability Against Diverse Interfering Substances

Figure 3. Performance of the NadPure Blood Genomic DNA Kit for blood gDNA extraction in the presence of various interfering substances. gDNA was extracted from simulated blood samples containing various endogenous and exogenous interfering substances using the NadPured Blood Genomic DNA Kit, and the quality of the extracted products was evaluated. A. DNA yield; B. DNA purity; C. Agarose gel electrophoresis of extracted gDNA.
Note: Based on relevant NMPA/FDA review and guidance documents and authoritative international literature, interfering substances were added at concentrations considered clinically relevant. For each group, 100 μL of whole blood was used as the initial sample, and the corresponding amount of each interfering substance was added.The volume was then brought to 200 μL with 1X PBS, followed by gDNA extraction according to the kit’s standard operating procedure. TG: Triglycerides; BIL: BilirubinTriglycerides: Hb: Hemoglobin; DC: Disodium citrate: SH: Sodium heparin; SIM: Simvastatin.

Consistent Performance Across Manufacturing Batches

Figure 4. NadPure Blood Genomic DNA Kit demonstrates good consistency in gDNA extraction from blood samples across different batches. A. DNA yield; B. DNA purity; C. Agarose gel electrophoresis of extracted gDNA.
Note: The initial sample input volume was 200 μL for all samples.

High-Quality Nucleic Acids for Downstream Applications

Figure 5. NGS targeted capture libraries prepared using gDNA extracted with the NadPure Blood Genomic DNA Kit demonstrate quality comparable to that of libraries prepared using other suppliers’ kits. A. Mappability and On-target rate; B. Target covered; C. Fold 80 base penalty; D. GC bias. gDNA was extracted from fresh whole blood samples using the NadPure Blood Genomic DNA Kit and Vendor A & B, respectively. Pre-libraries were prepared using the NadPrep EZ DNA Library Preparation Kit v3, followed by hybridization capture using the NadPrep ES Hybrid Capture Reagents v2 and the NanOnco Plus Panel v3.0. Raw reads were aligned to the human reference genome using BWA. On-target rate was calculated by the number of reads. Sequencing was performed on the NovaSeq 6000, PE150.

For research use only. Not for use in diagnostic procedures.

Package	Color of Cap Tube	Component	Volume 1010101 (24 rxn)
Box1	/	Blood-Lysis Buffer	7.5 mL
	/	Blood-Wash Buffer 1	8 mL
	/	Blood-Wash Buffer 2	2×4.5 mL
	/	Elution Buffer	2.5 mL
		Blood-Magnetic Beads	720 μL
Box 2		Proteinase K	480 μL

Package	Color of Cap Tube	Component	Volume 1010102 (96 rxn)
Box1	/	Blood-Lysis Buffer	30 mL
	/	Blood-Wash Buffer 1	35 mL
	/	Blood-Wash Buffer 2	2×18 mL
	/	Elution Buffer	10 mL
	/	Blood-Magnetic Beads	3 mL
Box 2		Proteinase K	1,000 μL

For research use only. Not for use in diagnostic procedures.

What are the possible reasons for low nucleic acid yield when using the NadPure Blood Genomic DNA Kit, and how to solve them?

•Samples: Improper blood sample storage, repeated freeze-thaw cycles, or prolonged storage may reduce DNA yield. It is recommended to storage and process blood samples under the recommended conditions and avoid repeated freeze-thaw cycles whenever possible.

•Reagents: Failure to add the required volumes of absolute ethanol to Blood-Wash Buffer 1 and Blood-Wash Buffer 2 may affect washing efficiency and the final DNA recovery yield. For the initial use, the absolute ethanol should be added to Blood-Wash Buffer 1 and Blood-Wash Buffer 2 according to the volumes indicated on the bottle labels. Vortex to mix thoroughly and label the buffers before use.

•Operations: After blood sample lysis, allow the reaction mixture to equilibrate to room temperature before sequentially adding isopropanol and Blood-Magnetic Beads. Mixing thoroughly to ensure sufficient binding of DNA to the beads. Avoid mixing the sample after adding isopropanol but before adding Blood-Magnetic Beads, as this may affect DNA binding to the beads.

Is the NadPure Blood Genomic DNA Kit suitable for hemolyzed samples or blood samples that have been stored for an extended period?

Yes. However, sample quality may affect DNA extraction performance. In general, fresh blood samples stored under appropriate conditions are more likely to get high-quality genomic DNA. Samples that are of poor quality or have been stored for prolonged periods may result in a low DNA yield and purity. For such samples, the extraction performance can be optimized by increasing the amount of Proteinase K or extending the lysis time.

Is the NadPure Blood Genomic DNA Kit suitable for concentrated blood samples obtained after plasma separation?

Yes. For a 100 μL concentrated blood sample, refer to the extraction procedure for a 200 μL blood sample in this user manual. If the actual sample volume differs, adjust the sample pretreatment procedure and the volumes of the relevant reagents according to the instructions for different sample volumes provided in this manual.

Is the NadPure Blood Genomic DNA Kit suitable for blood samples with larger input volumes?

The recommended standard input volume for this kit is 200 μL. For blood samples of other volumes, refer to the Protocol section of this user manual for specific processing requirements for different sample volumes. For blood input volumes of 200-1,000 μL, if a Red Blood Cell Lysis Buffer is unavailable, the validated pretreatment procedure can be used. For example, add 3X volumes of 1X PBS to the blood sample (e.g., add 900 μL of 1X PBS to 300 μL of blood sample). Mix thoroughly by inversion and centrifuge at 2,000 × g for 5 min. Carefully remove and discard the supernatant using a pipette. Add 200 μL of 1X PBS and vortex to mix thoroughly, then proceed with the Genomic DNA Extraction Workflow.