NadPrep NanoBlockers (for Illumina®, NXT) is a universal blocking sequencing designed for use with Illumina's sequencing platform for the Nextera™ library. This product can bind to the Tn5 adapters sequences of the libraries, reducing non-specific binding between adapters, which enhances on-target rate, increasing the depth of enrichmen
Single-tube universal: Capable of blocking adapter libraries with single or dual 6-10 nt indexes.
Efficient blocking: Significantly enhances the efficiency of captured data.
Stable performance: Excellent and consistent capture performance across different batches and sizes of panels.
User-friendly: Compatible with Nanodigmbio's liquid-phase hybrid capture system
Workflow
Performance
Figure 1. The blocking efficiency of NadPrep NanoBlockers
(for Illumina®, NXT). A total of 500 ng per library was used, with
overnight hybridization of 16 hours, in combination with NadPrep Hybrid Capture Reagents and panels of various sizes. Under
both single-plex (500 ng) and 12-plex (6000 ng) mixing schemes,
the performance of NadPrep NanoBlockers (for Illumina®, NXT)
remained excellent and stable across panels of different sizes. A. Mappability and on-target rate; B. Coverage uniformity and
consistency; C. Fold 80
For research use only. Not for use in diagnostic procedures.
No. They can effectively block a total library input of up to 6 µg during hybridization capture when starting with 500 ng/library and performing 12 multiplexing cycles.