NanOnco Plus Panel v3.0

Catalog Number:

#1001112F (16 rxn)
#1001111F (96 rxn)

NanOnco Plus Panel v3.0 targets the full coding regions from 620 genes (HLA genes included) of interest in solid tumor studies, a series of intron regions related to common fusions, classical microsatellite sequences, and chemotherapy-associated polymorphic loci. This panel involves a total number of 637 genes, covering an approximate 2.4 Mb target region of the genome. It supports the enrichment of multiple variants including base substitution, insertion/deletion, gene rearrangement, gene amplification, microsatellite instability and so on.

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Performance

Capture performance

Figure 1. Capture performance of NanOnco Plus Panel v3.0 in different gDNA libraries Libraries were prepared using NadPrep EZ DNA Library Preparation Kit coupled with NadPrep UDI Adapter (for Illumina®), and sequenced on Illumina Novaseq 6000 with PE150. The BWA was used for alignment to the reference genome hg38 and on-target rate was calculated by the number of reads. A. Mappability and on-target rate; B. Target covered; C. Coverage uniformity and consistency.
Note：The gDNA_1-4 samples were: Human Male Genomic DNA (Promega-male, G1471); Onco Structural Multplex 5% gDNA (Genewell, GW-OGTM001); Onco SNV Multiplex 1–25% gDNA (Genewell, GW-OGTM004); Onco SNV Wildtype gDNA (Genewell, GW-OGTM005), respectively.

Multiple variant analysis

Figure 2. Consistency of the allele frequencies in NanOnco Plus Panel v3.0 capture data with the nominal frequencies of the standardsLibraries were prepared using NadPrep EZ DNA Library Preparation Kit coupled with NadPrep Universal Stubby Adapter (UDI), and sequenced on Illumina Novaseq 6000 with PE150. Variant analysis were performed using Vardict.

Note：Samples were Onco Multiplex gDNA (LDTBIO, custom standards) with an average sequencing depth of 3,518x.

Table 1. Detection of other variant types using NanOnco Plus Panel v3.0.

Libraries were prepared using NadPrep EZ DNA Library Prepation Kit coupled with NadPrep UDI Adapter (for Illumina®), and sequenced on Illumina Novaseq 6000 with PE150. Variant analysis were performed using Vardict, Delly and CNVkit. The average sequencing depth of GW-OGTM001 and GW-OGTM005 is 966.71x and 1,126.04x, respectively.
Note：Note: The sample was Onco Structural Multplex 5% gDNA (GeneWell, GW-OGTM001); the reference was Onco SNV Wildtype gDNA (GeneWell, GW-OGTM005).

For research use only. Not for use in diagnostic procedures.

Catalog#	Color of Tube Cap	称Product	Volume	Package/Storage
1001112F		NanOnco Plus Panel v3.0, 16 rxn	70 μL	–20℃
1001111F		NanOnco Plus Panel v3.0, 96 rxn	415 μL	–20℃

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.