Simple and Convenient
Fast and Efficient
Flexible Compatibility
Workflow
Performance
Compatible with Different Input Amounts for Various Sample Types
Compatible with FFPE Samples of Different Quality Grades
Compatible with Third-party RNA Library Preparation Kits
Figure 3. Performance of the NadPrep rRNA Fast Blocking Comprehensive Solution when combined with a third-party RNA library preparation kit. A.rRNA ratio; B. No. of genes detected; Transcript coverage; D. Expression profile correlation. 250 ng of K562 cell line RNA was used with the NadPrep rRNA Blocking Reagent in conjunction with the NadPrep Total RNA-To-DNA Module coupled with the NadPrep DNA Universal Library Preparation Kit, the KAPA RNA HyperPrep Kit, and the NEBNext® Ultra II RNA Library Prep Kit for Illumina® to complete the library preparation.
Compatible with MGI and Illumina Sequencing Platform
Figure 4. Performance of the NadPrep rRNA Fast Blocking Comprehensive Solution on dual sequencing platforms.A. rRNA ratio; B. No. of genes detected. With an input of 250 ng, libraries were prepared using the NadPrep rRNA Fast Blocking Comprehensive Solution in combination with the NadPrep DNA Universal Library Preparation Kit, coupled with the NadPrep Universal Stubby Adapter (UDI) Module and NadPrep Universal Adapter (MDI) Module respectively. Sequencing platform: DNBSEQ-T7 and Illumina Novaseq 6000, PE150.
Competitive Comparison
Figure 5. Performance comparison between the NadPrep rRNA Fast Blocking Comprehensive Solution and competitors.A. rRNA ratio; B. No. of genes detected; C. Transcript coverage; D. Expression profile correlation. 250 ng of input, libraries were prepared using NadPrep rRNA Blocking Reagent, Vendor A, and Vendor B in combination with the NadPrep Total RNA-To-DNA Module.
Note: Vendor A refers to the rapid removal method, and Vendor B refers to the RNase H enzymatic method. C&D are shown only with the cell line (K562 RNA).For research use only. Not for use in diagnostic procedures.
RNA-To-DNA Module
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Package
Color of Cap Tube
Component
Volume
1002401 (24 rxn)
Volume
1002402 (96 rxn)
Box1
1st Strand Synth.
Buffer
120 µL
460 µL
Random Primer
30 µL
115 µL
1st Strand Synth.
Enzyme
60 µL
230 µL
2nd Strand Synth.
Buffer
810 µL
2×1550 µL
2nd Strand Synth.
Enzyme
90 µL
345 µL
Nuclease Free Water
2.5 mL
8 mL
Box2
NadPrep SP Beads
2.5 mL
10 mL
rRNA Fast Blocking Module
Volume
1002901 (24 rxn)
Volume
1002902 (96 rxn)
Color of Cap Tube
Component
rRNA Blocking Reagent (Human)
30 µL
115 µL
A: This solution is suitable for RNA samples from a wide range of cell and tissue sources with varying degradation levels. However, it is not recommended for severely degraded FFPE samples (DV 200 < 20%).
A: Yes, it is. This solution can be integrated with RNA library preparation kits for the preparation of total RNA sequencing (RNA-Seq) libraries. If you choose a third-party RNA library preparation kit, we recommend using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB, Cat # E7770L) or the KAPA RNA HyperPrep Kit (Roche, Cat # KK8541). For the use of RNA library preparation kits from other brands, please consult technical support from Nanodigmbio with support@njnad.com.
A: This solution contains a large amount of rRNA ssDNA blocking reagent. When used in conjunction with the NadPrep Total RNA-To-DNA Module, there may be a slight residual of ssDNA in the purified dsDNA product. When quantifying dsDNA using a nucleic acid fluorescence quantifier, this residual ssDNA can influence the results, leading to a slightly higher yield. However, the remaining rRNA ssDNA blocking reagent does not affect subsequent experiments. We recommend appropriate adapter dilution factors and PCR amplification cycles for different different types of RNA and different input amounts.
Type |
Product |
Detail |
Catalog# |
rRNA Fast Blocking Module |
NadPrep rRNA Blocking Reagent (Human), 24 rxn |
24 rxn |
1002901 |
NadPrep rRNA Blocking Reagent (Human), 96 rxn |
96 rxn |
1002902 |
|
Total RNA-To-DNA Module |
Nadprep Total RNA-To-DNA Module, 24 rxn |
24 rxn |
1002401 |
Nadprep Total RNA-To-DNA Module, 96 rxn |
96 rxn |
1002402 |
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