μCaler RP Panel v1.0

Catalog Number:

#1101431

μCaler RP Panel v1.0 is specifically designed to target the characteristic genes in the genomes of hundreds of respiratory pathogens (including RNA viruses, DNA viruses, bacteria, fungi, mycoplasma, and chlamydia). It targets over 99% of common respiratory infection pathogens, providing comprehensive support for multiplex pathogen co-detection, precise identification and typing, as well as variants tracking of influenza A virus. This panel facilitates the rapid and accurate diagnosis of complex respiratory infections while enhancing epidemiological surveillance and management.

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Pathogen Species List

Virus (34 species)	DNA virus (11 species 29 subtypes)	Human Polyomavirus 1 (BK Polyomavirus), Human Polyomavirus 2 (JC Polyomavirus), Human Polyomavirus 4 (WU Polyomavirus), Human Mastadenovirus B (3 /7/11/14/16/21/34/35/50/55 subtype), Human Mastadenovirus C (1/2/5/6/57 subtype), α Herpesvirus (1/2/3 subtype), γ Herpesvirus (4 subtype), β Herpesvirus (5/6/7 subtype), Human Bocavirus (1/2/3/4 subtype), Human Parvovirus B19, Monkeypox Virus
	RNA virus (23 species 203 subtypes)	Human Rhinovirus A (77 subtypes: 1-2, 7-13, 15-16, 18-25, 28-34, 36, 38-41, 43, 45-47, 49-51, 53-68, 71, 73-78, 80-82, 85, 88-90, 94-96, 98, 100-101, 103, 105-106), Human Rhinovirus B (25 subtypes: 3-6, 14, 17, 26, 27, 35, 37, 42, 48, 52, 69, 70, 72, 79, 83-84, 86, 91-93, 97, 99), Human Rhinovirus C (32 subtypes: 1-3, 5-9, 11-13, 15, 17-19, 22-24, 26, 30-33, 35, 40-44, 47, 53, 55), Human Respiratory Syncytial Virus (A/B subtype), Human Parainfluenza Virus (1/3 subtype), Human Parainfluenza Virus (2/4 subtype), Mumps Virus, Influenza A Virus (coding gene), Influenza B Virus, Coxsackievirus (24 subtypes: A1-A3, A5-A8, A10, A12-A22, B1, B3-B6), Echovirus (11 subtypes: E1, E3-E7, E9, E11, E25, E30, E32), Enterovirus (25 subtypes: E1, E3-7, E11, E25, E30, E32, A71, A90, A92, A120, A123, B69, C99, C105, C109, C113, C116, D68, D94, D111, J103), Human Metapneumovirus, Measles Virus (Genotype D8), Rubella Virus (Genotype 2B), SARS-CoV, SARS-CoV-2, MERS, Human Coronavirus (OC43, HKU1), Human Coronavirus (229E, NL63)
Bacteria (57 species)		Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Streptococcus pneumoniae, streptococcus dysgalactiae, Streptococcus intermadius, Streptococcus anginosus, Streptococcus constellatus, Streptococcus pyogenes, Streptococcus agalactiae, Peptostreptococcus anaerobius, Mycobacterium tuberculosis , Mycobacterium bovis, Mycobacterium africanum, Mycobacterium canetti, Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium lepromatosis, Mycobacterium asiaticum, Mycobacterium szulgai, Mycobacterium ulcerans, Mycobacterium xenopi, Mycobacterium scrofulaceum, Mycobacterium simiae, Mycobacterium novum, Mycobacterium haemophilum, Mycobacterium botniense, Mycobacterium kansasii, Mycobacterium abscessus, Mycobacterium chelonae, Neisseria gonorrhoeae , Neisseria meningitidis , Bacillus anthracis , Bacillus cereus, Arcanobacterium haemolyticum, Corynebacterium diphtheriae , Fusobacterium necrophorum, Haemophilus influenzae, Haemophilus parainfluenzae, Nocardia abscessus, Nocardia asteroides, Nocardia brasiliensis, Serratia marcescens, Moraxella catarrhalis, Bordetella pertussis, Bordetella parapertussis, Bordetella bronchiseptica, Bordetella holmesii, Legionella pneumophila, Elizabethkingia meningoseptica, Coxiella burnetii, Brucella abortus, Brucella anthropi, Brucella melitensis
Fungi (13 species)		Pneumocystis jirovecii, Cryptococcus neoformans, Cryptococcus gattii, Trichosporon, Mucor, Candida albicans, Candida tropicalis, Candida dubliniensis, Candida parapsilosis, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Aspergillus nidulans, Histoplasma capsulatum
Others (4 species)		Mycoplasmoides pneumoniae, Chlamydia pneumoniae, Chlamydia psittaci, Chlamydia trachomatis

*Species not identified.

Feature

Multiplex Pathogen Detection: Simultaneous detection of multiple pathogens: bacteria, viruses, fungi, mycoplasma, and chlamydia.
Precise Identification and Typing: Identification of 232 subtypes, including adenovirus, rhinovirus, parainfluenza virus, enterovirus etc.
Influenza A Virus Variants Tracking: Probes designed for the whole coding genes of the major 16 HA subtypes and 9 NA subtypes.

For research use only. Not for use in diagnostic procedures.

Click here for more details on the "μCaler Respiratory Pathogen Solution".

Catalog	Cap Color	Prodcut	Volume	Package/Storage
1101431		μCaler RP Panel v1.0, 96 rxn	210 μL	-25~-15℃

For research use only. Not for use in diagnostic procedures.

Can RNA samples be directly used for library preparation with this kit?

No. This kit is compatible only with gDNA or cDNA as initial samples. For RNA samples, reverse transcription to generate cDNA is required prior to library preparation.

What is the recommended input range? How to handle inputs exceeding this range?

This kit supports 50-2,000 ng of gDNA or cDNA. For input amount exceeds 2,000 ng, split the sample into multiple reactions to maintain amplification efficiency.

What is the insert size of this kit? How to select sequencing read length?

The main peak of PCR products by using this kit is ~270 bp. PE150 sequencing is recommended for high-quality coverage.

What is the recommended sequencing data volume for IGTR immune repertoire analysis?

A minimum of 0.3 Gb is recommended to detect clones at 0.01% abundance with an input of 200 ng. Increase data volume to enhance sensitivity for low-frequency clones.

Is this kit suitable for minimal residual disease (MRD) monitoring?

Yes. This kit includes IG Primer Mix and TR Primer Mix with gene-specific primers provided in separate tubes, allowing flexible combination in a single amplification reaction. With its high sensitivity, the kit meets the requirements for MRD monitoring technology development and clinical applications, making it ideal for low-frequency variant detection scenarios.